Figure 3
Selected co-induced lncRNA/mRNA pairs. (A) The genome browser screenshots of control cells (DMSO, upper panels) and PMA/ionomycin-treated cells (PMA/Iono, lower panels) show the RNA-seq data (strand + in yellow and strand − in red) as well as the ChIP-seq data (H3K4me3 in green and H3K27ac in blue) on the induced lncRNA loci and their nearby coding genes. Black arrows indicate the distance between the lncRNA and its neighboring gene. The primers used for RT-PCR validation are denoted by vertical red lines under the lncRNA position. (B) The PCR amplification of some lncRNAs that are induced by the PMA/ionomycin treatment are shown in control cells (DMSO), PMA/ionomycin-treated P5424 cells (PMA/Iono) and genomic DNA (gDNA). The Actb gene was used as an internal control in the experiments (Supplementary Fig. 3C).
