Figure 6
From: DNA polymerase ι is acetylated in response to SN2 alkylating agents
Effect of DNA-damaging agents on Polι acetylation in HEK293T cells. (a) Acetylation of Polι in HEK293T cells transfected with the FLAG-Polι expressing vector and treated, as indicated, with UV-irradiation – 7 J/m2, MMS – 1 h, zeocin – 12 hrs, hydrogen peroxide (H2O2) – 1 h, mitomycin C (MMC) – 40 hrs, cisplatin (cisPt) – 12 h, or hydroxyurea (HU). 10μg of protein extract was loaded in each line. Polι acetylation was visualized by western blot using monoclonal antibodies against acetylated lysines (KAc). Polι was visualized using polyclonal rabbit antibodies against a C-terminal fragment of Polι. (b) Acetylation of Polι in HEK293T cells transfected with FLAG-Polι plasmid and treated for 1 h with increasing doses of the oxidative agents, Hydrogen peroxide (H2O2) or Potassium bromate (KBrO3). (c) Acetylation of Polι in HEK293T cells transfected with the FLAG-Polι plasmid and treated for 1 h with alkylating agents, MMS, EMS, MNNG and DMS.
