Figure 7
From: DNA polymerase ι is acetylated in response to SN2 alkylating agents
Characterization of Polι K550R and K550Q variants. (a) Effect of the K550R substitution on MMS-induced Polι acetylation. Western blot using monoclonal antibodies against acetylated lysines. Polι was visualized using polyclonal rabbit antibodies against a C-terminal fragment of Polι. (b) Comparison of Polι localization into “replication factories” of wild-type GFP-Polι, GFP-Polι-K550R, and GFP-Polι-K550Q, respectively that block, or mimic, acetylation at the K550 residue. Respective plasmids were transfected into MRC5 cells. Histogram showing the percentage of nuclei with foci (left panel). Numbers are based upon 3 replicates where 200 nuclei were counted for each cell line from GFP positive cells. Example of GFP-positive cells with a nucleus without foci (right top panel), or with foci (right bottom panel).
