Figure 2
From: Glutathione-related substances maintain cardiomyocyte contractile function in hypoxic conditions
Changes in the concentration of free intracellular Ca2+ in isolated rat cardiomyocytes during electric stimulation (1 Hz) under hypoxic conditions (buffer saturated with 95% N2 and 5% CO2) in the presence of low molecular weight thiols. 10 sec fragments of the records in the presence of 0.5 mM et-GSH, 0.5 mM GSSG, 0.5 mM tet-GSSG, GSNO and 10 mM NAC, at 3 min of hypoxia are given. Similar recordings were obtained during the next 8–12 minutes of hypoxia depending on the substance added. The ordinate shows the fluorescence intensity of Fluo-4 (a.u.).
