Figure 6
From: Glutathione-related substances maintain cardiomyocyte contractile function in hypoxic conditions
S-glutathionylation of α1 and α2 isoforms of Na,K-ATPase catalytic-subunit after GSNO treatment of cardiomyocytes. α1-Subunit (A) or α2-subunit (B) of Na,K-ATPase was immunoprecipitated (IP) from cell lysates by anti-α1 antibodies or anti-α2 antibodies and glutathionylation was detected with anti-glutathione (anti-GS) antibodies. The original immunoblotting readouts are presented above. Bars represent changes in the S-glutathionylated (GSS- α1/α1) or (GSS- α2/α2) form of the protein normalized to its total amount; n = 3, mean ± SD; *p < 0.05. Full-length blots are included in Supplementary materials (Supplementary Fig. 7).
