Figure 10 | Scientific Reports

Figure 10

From: Non-canonical WNT-signaling controls differentiation of lymphatics and extension lymphangiogenesis via RAC and JNK signaling

Figure 10

Tube-formation-assay with HD-LECs after pre-treatment with 10 µM LGK974 for 3 days and subsequent application of 500 ng recombinant WNT5A. (A–C) Representative images of tube-formation-assay with DMSO-controls, HD-LECs pre-treated with LGK974 for 3 d and subsequent application of recombinant WNT5A. Evaluation was performed 9 h after cell seeding. Bar graphs show (D) the cell‐covered area, (E) number of tubes, (F) total tube length, and (G) number of branching points. Note the highly significant increase in all aspects of tube formation by WNT5A.

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