Figure 8
Ex vivo treatment of dorsal thoracic dermis from ED 15.5 Wnt5a−/− mice with recombinant WNT5A protein for 2 days. Typical results after staining with anti-Lyve1 antibodies. (A–C) Control-treated specimens show an immature vascular plexus. (D–F) WNT5A induced vessel extension and more mature network formation. Bar = 50 µm. (G,H) Quantification of WNT5A effects reveals significantly decreased vessel-covered area (Mann-Whitney test, p = 0.0079), without statistically significant decrease of the number of branching points (Mann-Whitney test, p = 0.1425). (I,J) Higher magnifications of (C,F), respectively, showing wide sweeping morphology of LECs in control-treated specimens (I), and longitudinally oriented LECs in WNT5A-treated dermis (J). Bar = 15 µm.
