Figure 9
Ex vivo treatment of dorsal thoracic dermis from ED 15.5 wild-type-mice with LGK974 for 2 days. Typical results after staining with anti-Lyve1 antibodies. (A–C) WT-controls; (D–F) Application of 50 µM LGK974. Bar = 50 µm. Note the increase in vessel-covered area (G) and number of branching points (H) after 50 µM LGK974, indicating a failure of network maturation. (t-test, LGK974 50 µM p = 0.0004 in (G) Mann-Whitney test, LGK974 50 µM p = 0.0244 in (H).
