Figure 1 | Scientific Reports

Figure 1

From: Noninvasive Contrast-Free 3D Evaluation of Tumor Angiogenesis with Ultrasensitive Ultrasound Microvessel Imaging

Figure 1

Functional imaging of CAM engrafted cell-line tumors. (A) Overhead view of an ex ovo chicken embryo model. Cancer cell lines engrafted into the CAM resulted in highly vascularized, solid, and spheroidal tumor masses. (B) H&E section taken from representative control tumor (DMSO treated). High magnification section reveals viable tumor nuclei with a high degree of adjacent vasculature. (C) Endpoint tumor volumes for each treatment group. Differences in volume were not found to be significant. Data are mean ± s.d. (D) Schematic representation of tumor imaging protocol. Tumors were imaged throughout their volume with a step size of 0.25 mm. (E) Representative image of control (DMSO) treated Renca tumor obtained with conventional power Doppler of VisualSonics Vevo® 3100. (F) SVD-based clutter filtering of ultrasound echoes from the tumor in (E) resulted in improved vascular imaging (Supplementary Video S1). (G) Fluorescent histology section of the example tumor, which confirms high intratumoral vascularization (red signal).

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