Figure 3
UMI improved detection and quantification of anti-angiogenic treatment effect. (A) Control tumor exhibited a high degree of intratumoral vascularization in conventional power Doppler imaging and UMI (Supplementary Video S2). H&E histology confirmed viable tumor cells and the presence of dense capillary networks. Fluorescent histology permitted the quantification of MFI and fluorescent vessel area as surrogate measures of microvascular density. (B) Conventional power Doppler imaging of pazopanib treated tumor did not reveal a significant change in vascularization. UMI image revealed localized avascular regions. Histology revealed some regional necrosis and a slightly reduced microvascular density (Supplementary Video S3). (C) Sunitinib treated tumor exhibited a modest decrease in tumor vascularization on Vevo®’s conventional Doppler image. This anti-angiogenic effect was more pronounced in UMI image (Supplementary Video S4). Histology confirmed intratumoral necrosis and a reduced microvascular density. Scale bar is 1 mm. Refer to Supplementary Fig. S1 and supplemental videos for 3D images. Rotation animations of the volumetric renderings are displayed in Supplementary Video S5.
