Figure 3 | Scientific Reports

Figure 3

From: The putative tumour suppressor protein Latexin is secreted by prostate luminal cells and is downregulated in malignancy

Figure 3

LXN reduces the plating efficiency of prostate luminal cells. (A) Representative image from a Western blot (n = 4) showing robust stable overexpression of LXN protein in prostate epithelial cell lines following lentiviral transduction. See Supplementary Fig. S9 for full length uncropped blot images (B). Cell viability assays demonstrating the effects of LXN overexpression in non-malignant (PNT1A and BPH-1) and malignant (P4E6 and LNCaP) prostate epithelial cell lines. (C–H) The effects of low (10 Gy) and high (75 Gy) doses of γ-irradiation on the viability (C,E,G) and apoptosis (D,F,H) of prostate epithelial cell lines. (I,J) Wound scrape assays to determine the effects of LXN overexpression on wound healing and motility in BPH-1 and PNT1A cell lines. (K) Effect of LXN overexpression on the colony forming effeciency of primary prostate epithelial data representative of 3 patient samples (L). Plating efficiency assays in prostate epithelial cell lines (n = 3). NS = non-significant. p = 0.0013.

Back to article page