Figure 3
(a) Amplitude map of Objx, and Objy, amplitude parameter β, phase contrast for Objx and Objy, phase difference Δϕ relative to a control sperm cell (0 h in heparin). Colorbars indicate the mapping of the phase variation. The Δϕ map shows a birefringence distribution (bright pattern) over all the sperm head. (b) The same holographic parameters measured for a reacted sample (4 h in heparin). The Δϕ map after 4 h in heparin shows a clear decrease of the birefringent area. In particular, the birefringence (bright pattern) is confined in the post-acrosomal region. (c) Distribution of birefringence patterns of sperm from 3 donors exposed to heparin for 0 h (control sample), and 4 h (reacted sample). Scale bar: 4 μm.
