Figure 8

(a) Mean Raman spectra from the AA and ES sperm cell regions comparing control sample (0 h) and capacitated sperm cells (4 h in heparin). (b) I2850/I2880 and I2880/I2930 ratio variation in capacitated sperm compared to the control sample in the AA and ES regions. (c) 3D PCA scatter plots of the first three principal components for each the sperm cell region exposed to heparin for 0 (ctr) and 4 h. The Raman spectra corresponds to cells preliminarily selected by DHP analysis.