Figure 9

Citral induced apoptosis in AGS cell lines. (A) DNA condensation and nuclear staining of AGS cells with or without citral treatment. (B) Flow cytometry analysis of cancer cells with or without treatment. Expressive figures displaying the population of live cells (annexin V− PI−), early apoptotic (annexin V+ PI−), late apoptotic cells (annexin V+ PI+) and necrotic cells (annexin V− PI+). (C) PI staining of AGS cells with or without citral treatment. The cells remained intact don’t allow cells to stain with the dye. However, the damage cells were stained with PI and indicated the apoptotic cells. (D) DNA fragmentation analysis was carried out after isolation of genomic DNA from AGS with or without different concentration of citral treatment. 1. DNA ladder 1 kb; 2. Control; 3. 5 µg/mL; 4. 10 µg/mL; 5. 20 µg/mL.