Table 1 Cycle threshold (CT) values obtained during qPCR of the pfcrt haplotypes CVMNK and CVIET in the 3D7 and Dd2 P. falciparum strains, respectively), following manual (chelex) or robotic (QIAsymphony) extraction.
From: Molecular quantification of Plasmodium parasite density from the blood retained in used RDTs
Format | Method* | DNA dilution (p/μL) | 3D7 CVMNK | Dd2 CVIET | ||||
|---|---|---|---|---|---|---|---|---|
Mean Ct | Ct STD | Ct CI | Mean Ct | Ct STD | Ct CI | |||
Manual | Chelex | 1:1 (50000) | 22.86 | 0.34 | 22.58–23.14 | 23.10 | 0.28 | 22.87–23.33 |
1:10 (5000) | 25.91 | 0.74 | 25.31–26.51 | 25.61 | 0.56 | 25.15–26.07 | ||
1:100 (500) | 27.92 | 0.36 | 27.63–28.21 | 27.45 | 0.34 | 27.17–27.73 | ||
1:1000 (50) | 30.28 | 0.26 | 30.07–30.49 | 30.35 | 0.31 | 30.10–30.60 | ||
1:5000 (5) | 35.94 | 0.47 | 35.56–36.32 | 36.21 | 0.53 | 35.78–36.64 | ||
1:10000 (1) | 38.02 | 2.00 | 36.39–39.65 | 38.60 | 1.89 | 37.06–40.14 | ||
Automatic | Investigator | 1:1 (50000) | 21.8 | 0.30 | 21.56–22.04 | 22.72 | 0.49 | 22.32–23.12 |
1:10 (5000) | 25.28 | 0.02 | 25.26–25.30 | 26.79 | 0.10 | 26.71–26.87 | ||
1:100 (500) | 26.3 | 0.42 | 25.96–26.64 | 29.76 | 0.47 | 29.38–30.14 | ||
1:1000 (50) | 30.61 | 1.14 | 29.68–31.54 | 29.60 | 0.37 | 29.30–29.90 | ||
1:5000 (5) | 35.51 | 0.52 | 35.09–35.93 | 35.21 | 0.26 | 35.00–35.42 | ||
1:10000 (1) | 36.72 | 1.91 | 35.16–38.28 | 36.80 | 1.86 | 35.28–38.32 | ||
Blood | 1:1 (50000) | 21.2 | 0.11 | 21.11–21.29 | 22.50 | 0.12 | 22.40–22.60 | |
1:10 (5000) | 25.28 | 0.17 | 25.14–25.42 | 26.51 | 0.24 | 26.31–26.71 | ||
1:100 (500) | 27.99 | 0.09 | 27.92–28.06 | 29.60 | 0.46 | 29.22–29.98 | ||
1:1000 (50) | 30.75 | 0.09 | 30.68–30.82 | 29.62 | 0.23 | 29.43–29.81 | ||
1:5000 (5) | 35.39 | 0.78 | 34.75–36.03 | 35.31 | 0.81 | 34.65–35.97 | ||
1:10000 (1) | 36.72 | 1.91 | 35.16–38.28 | 36.40 | 1.86 | 34.88–37.92 | ||
