Figure 2 | Scientific Reports

Figure 2

From: Targeting Modified Lipids during Routine Lipidomics Analysis using HILIC and C30 Reverse Phase Liquid Chromatography coupled to Mass Spectrometry

Figure 2

(a) HILIC-MS chromatogram of modified lipids showing separation and comparisons of PE, P-PE, MMPE, DMPE, PG, PC, O-PC, P-PC and SM lipid classes in negative ion mode. (b–i) HILIC-MS/MS spectra obtained from the complex lipid standard mix: (b) PE 18:0/20:4 [M-H]− at m/z 766.54, (c) plasmalogen PE P-18:0/20:4 [M-H]− m/z at 750.54, (d) MMPE 16:0/16:0 [M-H]− at m/z 704.52, (e) DMPE 16:0/16:0 [M-H]− at m/z 718.54, (f) PC 18:0/20:4 [M + CH3COO]− at m/z 818.59, (g) plasmalogen PC P-18:0/20:4 [M + CH3COO]− at m/z 852.61, (h) SM d18:1/18:0 [M + CH3COO]− at m/z 789.61, and (i) diether PC O-18:0/O-18:0 [M + CH3COO]− at m/z 820.68.

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