Table 2 Relative composition of major (acyl)phosphatidylglycerol molecular species present in silage corn roots following cultivation in cool climatic conditions.

From: Targeting Modified Lipids during Routine Lipidomics Analysis using HILIC and C30 Reverse Phase Liquid Chromatography coupled to Mass Spectrometry

m/z

Lipid Ion

Molecular species

Di/triacyl species

% nmol

765.52

[M + Na-2H]−

PG 34:3

16:0/18:3

6.22 ± 0.20

767.53

[M + Na-2H]−

PG 34:2

16:0/18:2

53.43 ± 0.84

789.52

[M + Na-2H]−

PG 36:5

18:2/18:3

2.36 ± 0.25

791.53

[M + Na-2H]−

PG 36:4

18:2/18:2

9.29 ± 0.26

795.56

[M + Na-2H]−

PG 36:2

18:2/18:0

3.49 ± 0.08

1003.75

[M + Na-2H]−

acylPG 50:3

16:0/18:3-(16:0)

1.19 ± 0.07

1005.76

[M + Na-2H]−

acylPG 50:2

16:0/18:2-(16:0)

17.99 ± 0.50

1029.76

[M + Na-2H]−

acylPG 52:4

18:2/18:2-(16:0), 16:0/18:2-(18:2)

4.12 ± 0.28

1031.77

[M + Na-2H]−

acylPG 52:3

18:1-18:2-(16:0),16:0-18:2-(18:1),

1.91 ± 0.14

   

16:0-18:1-(18:2)

 
   

Total% acylPG

25.22 ± 0.80

   

Total% PG

74.78 ± 0.80

   

Total%

100

  1. Values (percent by relative peak area) represent means ± standard errors, n = 4 per experimental replicate. PG represents phosphatidylglycerol, acylPG represents acylphosphatidylglycerol subclass. The lipid components in the table are arranged based on m/z of the molecular species [M + Na-2H]− ions with the number before the colon representing total number of carbons, the numbers after the colon representing the total number of double bonds of all the fatty acyl chains composition (e.g., acylPG 50:2 represents acylPG molecules with 50 carbons and 2 double bonds on three acyl chains). The position of fatty acyl chains on glycerol backbone was denote with the order sn-1/sn-2-(sn-3′), hyphen was used if sn-1 and sn-2 were not assigned. Analysis was conducted using HILIC-HRMS/MS in negative ion mode.
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