Table 1 Characteristics of seven collection sites in Brazil and sample size of Ny. darlingi tested in laboratory for life history traits at each of three temperatures (total n = 2,652).

Biome	State	Locality	Latitude	Average mean temp (min, max)*	No. mosquitos at each temp.
Biome	State	Locality	Latitude	Average mean temp (min, max)*	20 °C	24 °C	28 °C
Amazon	Amazonas	ARS	−2.864	27.6 (26.9, 28.6)^a	134	135	130
	Amazonas	APR	−3.028	27.7 (26.7, 29.2)^a	198	198	179
	Rondônia	RPV	−8.742	25.7 (23.2, 26.6)^b	181	184	141
	Rondônia	RMO	−9.223	25.6 (22.8, 26.5)^b	192	197	160
Cerrado	Tocantins	TLC	−10.7	28.4 (23.3, 35.4)^a	92	84	71
Cerrado	Tocantins	TPN	−10.796	27.5 (26.2, 28.6)^a	32	35	24
Mata Atlantica	Rio de Janeiro	SJU	−22.611	21.2 (20.5, 22.3)^c	129	123	33

*Temperature data are means and ranges of annual mean temperatures (2010–2016) for local weather stations^84,85. Letters to the right of average mean temperatures are derived from Tukey HSD after ANOVA and indicate significant differences in temperatures among Localities (p-value < 0.01).

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