Figure 5
From: The Rice Phosphate Transporter Protein OsPT8 Regulates Disease Resistance and Plant Growth
Interaction between OsPT8 and BWMK1. (A) OsPT8 interacts with BWMK1 in yeast. SD/-Leu-Trp-His medium containing 35 mM 3-amino-1,2,4-triazole (3′AT) was used to test the interaction; (B) The in vitro GST pull-down assay confirms the interaction between OsPT8: MBP and BWMK1: GST. MBP was used as a negative control. The amounts of proteins were examined by Western immunoblot analysis with anti-MBP or anti-GST antibody. These blots were exposed on different gels; (C) LCI assay show that OsPT8 interacts with BWMK1 in N. benthamiana leaves. SGT1/RAR1 was positive control.OsPT8/EV and BWMK1/EV were negative controls.
