Table 1 Point mutation design.
From: Computer aided protein engineering to enhance the thermo-stability of CXCR1- T4 lysozyme complex
Mutation | Segment | Interacting residues | Rationale for mutation |
|---|---|---|---|
L812.46A | TM2 | M611.54, L1273.42 | Helical conformation and packing |
L1263.41W | TM3 | P2145.50 | Distance between L1263.41 and P2145.50 |
K1544.43A | TM4 | None | Increase hydrophobicity in TM region |
A2406.33D | TM6 | R1353.50 | Interact with DRY motif in TM3 |
G2947.32A | TM7 | W2556.48 | Interact with CWxP motif in TM6 |
