Figure 5 | Scientific Reports

Figure 5

From: Synthetic standard aided quantification and structural characterization of amyloid-beta glycopeptides enriched from cerebrospinal fluid of Alzheimer’s disease patients

Figure 5

PRM MS2 of desialylated Aβ1-15 glycopeptides and standard 1. (A) A SIM acquisition of the co-eluting precursors Galβ3GalNAc-Aβ1-15 (m/z 731.31) and 1 (m/z 733.31), corresponding to Route 2 (Fig. 2B) using QC CSF, and (B) their extracted ion chromatograms. (C) Full range MS2 spectrum of the two precursor ions. The inserts show m/z expansion of the peptide ions and b-ions demonstrating the 5 u mass difference between the peptide b-fragments of Galβ3GalNAc-Aβ1-15 and 1. (D) Expansion of the m/z 100–220 region showing the oxonium ions from the native glycopeptide marked a and the isotope labeled ions from 1 are marked *.

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