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Figure 1

From: Simulated microgravity promotes the formation of tridimensional cultures and stimulates pluripotency and a glycolytic metabolism in human hepatic and biliary tree stem/progenitor cells

Figure 1

Viability of tridimensional hBTSCs cultures in Kubota’s Medium (KM) and in hormonally defined medium (HDM) both in simulated microgravity (SMG) and in normal gravity condition (NG). hBTSCs viability was analyzed at day 0, at 7 days and at 14 days. The number of alive cells significantly decreased in SMG at 7 and 14 days when they were cultured in absence of an adhesion substrate (§p < 0.01; n = 3) (a). No significant differences in cells number were observed when hBTSCs were cultured in presence of Cytodex 3 microcarriers in SMG compared to controls in NG. Cytodex 3 microcarriers provided a proper and inert surface to grant adhesions signals that are necessary to ensure epithelial cell growth (b). Bright field image of hBTSCs grown on Cytodex 3 in SMG at 14 days (10x) (c). hBTSCs in presence of Cytodex 3 formed naked-eyes visible tridimensional cultures in a vessel at 14 days of culture (d).

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