Figure 2 | Scientific Reports

Figure 2

From: The Role of AMPARs in the Maturation and Integration of Caudal Ganglionic Eminence-Derived Interneurons into Developing Hippocampal Microcircuits

Figure 2

Decreased AMPAR-mediated transmission increases interneuron hippocampal invasion and differentially influences VIP- and CCK-containing interneuron survival. (A) Confocal images of hippocampi from WT and GluA1-3 KO mice at P0, P5 and P21. tdTomato fluorescent signal was used as a proxy to identify 5HT3AR-expressing CGE-derived interneurons. Fluorescent cells populate all layers of CA1 in hippocampus together with CA3 and dentate gyrus (DG) regions. Bar graph of cell density quantification performed on CA1 regions on mouse hippocampi. (B) Confocal images of hippocampi from WT (top row) and GluA1-3 KO (bottom row) mice at P5 show representative tdTomato expressing (left) CGE interneuron soma immunopositive for cleaved caspase 3 (CC3) (middle). Colocalization of tdTomato and CC3 signals are shown in the merged images (right). Bar graphs of cell quantification performed for CC3 positive CGE-derived interneurons shown as the density of CC3+ tdTomato+ cells and the fraction (%) of CC3+ cells in tdTomato+ population in the entire hippocampi. (C,D) Confocal images of hippocampi from WT and GluA1-3 KO mice at P20-P22 immunolabeled against VIP (C) and CCK (D) interneuron markers. Bar graphs of cell quantification performed on CA1 hippocampus for interneuron subgroups. *p < 0.05; **p < 0.005.

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