Figure 3
Higher accumulation of nPt in autophagy-deficient cells than in autophagy-normal cells. (a) The schedule of nPt administration; experiment 1 (upper) shown in (b,c), 4a,4b was to examine the uptake of nPt into the cells, and experiment 2 (lower) shown in (d) was to examine the retention of nPt in the cells. For experiment 2, cells cultured with nPt for 24 h were washed with PBS and fresh media was added. Then, intracellular nPt concentrations were measured at 12, 24, or 48 h. (b,c) The graph showed the intracellular nPt concentrations in the HchEpC1b-ATG4BC74A cells (black bars), the autophagy-deficient cells, and HchEpC1b-mStrawberry cells (white bars), the autophagy-normal cells, cultured with nPt at the indicated concentrations (μg/ml) for 24 h (b) or with 25 μg/ml nPt at the indicated time (c). (d) Intracellular nPt concentrations were measured in the HchEpC1b-ATG4BC74A cells (black bars) or HchEpC1b-mStrawberry cells (white bars) cultured with 25 μg/ml nPt for 24 h or at the indicated time since media was replaced. Data are expressed as the mean ± S.D. *p < 0.05, **p < 0.01, ***p < 0.001.
