Figure 4
Sequestration of nPt in the autophagosome-rich fraction. (a) Cell lysates of HchEpC1b-mStrawberry (Hch-mSt) cells or HchEpC1b-ATG4BC74A (Hch-4B) cells cultured with 25 μg/ml of nPt (+) or (−), PBS, were separated into fraction A (Fra: A), which mainly contained the cytoplasm, and fraction B (Fra: B), which mainly contained autophagosomes. (b) The graph showed the intracellular nPt concentrations in fraction A and B of HchEpC1b-mStrawberry cells and HchEpC1b-ATG4BC74A cells cultured with 25 μg/ml of nPt for 24 h. The nPt concentrations were normalized to that in fraction A of HchEpC1b-mStrawberry cells as 100%. Data were expressed as the mean ± S.D. *p < 0.05.
