Figure 5
Autophagy protected cells from nPt-mediated DNA damage. (a) Representative panels showed the nuclei staining (DAPI, blue), single staining of anti-phospho-γH2AX (γH2AX, green), and the merged images of the HchEpC1b-mStrawberry (Hch-mSt) cells or HchEpC1b-ATG4BC74A (Hch-4B) cells cultured with 12.5 μg/ml or 50 μg/ml of nPt for 24 h. (b) The average intensity of anti-γH2AX staining in each cell was shown in the graph. White bars indicated the Hch-mSt cells and black bars indicated Hch-4B cells. (c) The proportion of propidium iodide (PI)-positive Hch-mSt cells (white bars) or Hch-4B cells (black bars), which were cultured with 12.5 μg/ml or 50 μg/ml of nPt for 24 h, was shown. (d) Western blots of Hch-mSt or -4B cells, which were cultured with 12.5 or 50 μg/ml of nPt for 24 h, were as follows: γH2AX, and ACTB. (e) The graph showed the nPt concentrations in cytoplasmic (white bars) or nuclear (black bars) fraction in Hch-mSt or -4B cells treated with 12.5 μg/ml of nPt for 24 h. Data are expressed as the mean ± S.D. *p < 0.05, **p < 0.01, ***p < 0.001. Scale bars: 15 μm.
