Figure 7
Heat map and hierarchical clustering of digital gene expression of LusHSF genes in different flax tissues. (a) LusHSF corresponding gene IDs were derived from the microarray data under GEO accession no. GSE21868. The mean of RMA-normalized, averaged gene-level signal intensity (log2) values were plotted using the Heatmap Illustrator (HemI v.1.0). Tissue includes SIV: stem inner tissue from the vegetative stage; SOV: stem outer tissue from the vegetative stage; root; leaf; SIGC: inner stem at the green capsule stage; SOGC: outer stem at the green capsule stage; and embryo at 10, 20, and 40 days post flowering. (b) The normalized signal intensity values of the LusHSF genes derived from the transcriptome data under GEO accession no. GSE61311 is plotted as a heat map. Digital samples include WT-SIV: inner stem tissue from the vegetative stage of wild-type plants; mut-SIV: inner stem tissue from the vegetative stage of lignified bast fibre mutant plants; WT-SOV: outer stem tissue from the vegetative stage of wild type plant; and mut-SOV: outer stem tissue from the vegetative stage of lignified bast fibre mutant plant. (c) Heat map generated for the LusHSFs derived from RNA-seq data (Accession no. GSE80718) using the log2 transformed average FPKM values. In all heat map plots, the coloured bars shown on the right represent their expression levels.
