Table 1 Features of LusHSF genes and proteins in the flax genome.

From: Genomic insights into HSFs as candidate genes for high-temperature stress adaptation and gene editing with minimal off-target effects in flax

HSF ID	Phytozome	HSF	Location			Strand	Length			pI	GRAVY	Mw (kD)	Subcel loc and no. NN
HSF ID	locus	group	Chr*	Start*	End*	Strand	Gene	CDS	Protein	pI	GRAVY	Mw (kD)	Subcel loc and no. NN
LushsfA1a	Lus10011065	A1a	Lu08	18101211	18103152	reverse	1941	1377	458	4.82	−0.578	50.06	nu: 14
LushsfA1b	Lus10040911	A1b	Lu15	8448889	8450468	forward	1579	1377	458	5.65	−0.583	50.64	nu: 14
LushsfA1c	Lus10000312	A1c	Lu08	16643985	16645926	reverse	1941	1377	458	4.95	−0.602	50.01	nu: 14
LushsfA1d	Lus10030956	A1d	Lu09	5719143	5722728	reverse	3585	1416	471	4.90	−0.778	52.43	nu: 14
LushsfA1f	Lus10040091	A1f	Lu07	4032070	4035485	forward	3415	1416	471	4.72	−0.770	52.11	nu: 14
LushsfA2a	Lus10013797	A2a	Lu11	2671577	2672762	reverse	1185	1026	341	5.01	−0.682	38.43	nu: 14
LushsfA2b	Lus10039134	A2b	Lu14	18116147	18117412	forward	1265	1110	369	5.22	−0.649	41.46	nu: 13, cp: 1
LushsfA3a	Lus10023866	A3a	Lu08	4723718	4725601	forward	1883	1326	441	4.73	−0.570	49.81	nu: 14
LushsfA3b	Lus10014369	A3b	Lu06	14835072	14836968	forward	1896	1341	446	4.71	−0.561	50.31	nu: 14
LushsfA4a	Lus10007318	A4a	Lu15	2083596	2084949	reverse	1353	1242	413	4.97	−0.648	46.38	nu: 14
LushsfA4b	Lus10015237	A4b	Lu12	2940951	2942258	reverse	1307	1212	403	5.75	−0.880	45.68	nu: 14
LushsfA4c	Lus10029269	A4c	Lu03	8165193	8166105	reverse	912	801	266	6.01	−0.623	30.58	nu: 13, cp: 1
LushsfA4d	Lus10005420	A4d	Lu07	17858515	17860349	forward	1834	1473	490	7.92	−0.602	55.15	nu: 5, cp: 1, cy: 2, mt: 2, vac: 2, er: 1, gb:1
LushsfA6a	Lus10006618	A6a	Lu08	22068530	22070272	forward	1742	1185	394	5.56	−0.734	44.47	nu: 14
LushsfA6b	Lus10039376	A6b	Lu10	3252307	3253927	forward	1620	1122	373	5.11	−0.650	42.35	nu: 13, cp: 1
LushsfA7a	Lus10000492	A7a	Lu14	4577433	4578881	forward	1448	1125	374	5.52	−0.737	42.24	nu: 13
LushsfA7b	Lus10014698	A7b	Lu01	26411220	26412688	reverse	1468	1137	378	5.51	−0.780	42.96	nu: 14
LushsfA7c	Lus10022083	A7c	unmapped	-	-	reverse	1410	1131	376	5.75	−0.744	43.11	nu: 14
LushsfA8a	Lus10003707	A8a	Lu03	4843561	4845792	reverse	2231	1122	373	4.97	−0.703	42.42	nu: 13, per: 1
LushsfA8b	Lus10001591	A8b	Lu04	8648138	8650354	reverse	2216	1122	373	4.78	−0.724	42.72	nu: 13, per: 1
LushsfA9a	Lus10027627	A9a	Lu11	14143005	14144526	forward	1521	1422	473	5.03	−0.697	53.45	nu: 14
LushsfA9b	Lus10011941	A9b	Lu10	7901682	7903160	reverse	1478	1377	458	5.19	−0.696	51.76	nu: 14
LushsfB1a	Lus10019348	B1a	Lu02	1244140	1247030	forward	2890	273	822	4.81	−0.995	30.15	nu: 14
LushsfB1c	Lus10009351	B1c	Lu13	5055375	5058490	forward	3115	852	283	4.84	−0.991	31.44	nu: 11, cp: 1, cy: 1, pm: 1
LushsfB2a	Lus10014994	B2a	Lu14	19043189	19044240	forward	1051	924	307	5.58	−0.694	33.51	nu: 14
LushsfB2b	Lus10008007	B2b	Lu07	6384378	6385552	reverse	1174	1098	365	4.80	−0.554	39.28	nu: 13, per: 1
LushsfB2d	Lus10024508	B2d	Lu09	7452072	7454461	reverse	2389	1227	408	5.27	−0.499	44.34	nu: 13, per: 1
LushsfB4a	Lus10042646	B4a	Lu09	17698210	17699546	reverse	1336	1233	410	8.25	−0.656	45.07	nu: 14
LushsfB4b	Lus10036062	B4b	Lu01	503457	505257	reverse	1800	951	316	6.21	−0.899	36.54	nu: 14
LushsfB4c	Lus10001133	B4c	Lu10	13207020	13208344	reverse	1324	1215	404	7.84	−0.704	44.83	nu: 14
LushsfB5a	Lus10011185	B5a	Lu15	837963	839813	forward	1850	615	204	9.32	−0.812	23.78	nu: 13.5, cy_nu: 0.5
LushsfB5b	Lus10018488	B5b	Lu08	23772105	23774084	reverse	1979	603	200	9.27	−0.668	23.19	nu: 12.5, cy_nu: 7, cp 1
LushsfC1a	Lus10023636	C1a	Lu11	15833912	15834932	forward	1020	921	306	7.78	−0.715	34.63	nu: 14
LushsfC1b	Lus10034907	C1b	Lu12	8134272	8135285	forward	1013	924	307	6.98	−0.695	34.46	nu: 14

Chromosomal location estimated as per You et al.¹⁰.
pI: isoelectric point, GRAVY: Grand average of hydropathicity, Mw(kD): Molecular weight in kilo Dalton, Subcel loc and no. NN: Subcellular location and number of nearest neighbours, nu: nucleus, mt: mitochondria, cp: chloroplast, per: peroxisome, cy: cytosol, vac: vacuole, ER: endoplasmic reticulum, gb: golgi body, cy_nu: dual localization cytosol and nucleus, pm: plasma membrane.

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Table 1 Features of LusHSF genes and proteins in the flax genome.

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