Table 3 Details of DNA binding site predictions on LusHSF proteins.

From: Genomic insights into HSFs as candidate genes for high-temperature stress adaptation and gene editing with minimal off-target effects in flax

HSF_ID

No of contact sites

No of contact sites in N-ring

N-ring contact sites (position and amino acid)

Specificity

Entropy

PDB_ID of model

LushsfA1a

22

6

93-S, 96-R, 97-Q, 99-N, 100-T, 131-R

0.27

0.73

5d5v_B

LushsfA1b

22

6

63-S, 66-R, 67-Q, 69-N, 70-T, 101-R

0.27

0.87

5d5v_B

LushsfA1c

22

6

93-S, 96-R, 97-Q, 99-N, 100-T, 131-R

0.27

0.81

5d5v_B

LushsfA1d

22

6

97-S, 100-R, 101-Q, 103-N, 104-T, 135-R

0.27

0.83

5d5v_B

LushsfA1f

22

6

97-S, 100-R, 101-Q, 103-N, 104-T, 135-R

0.27

0.86

5d5v_B

LushsfA2a

22

6

97-S, 100-R, 101-Q, 103-N, 104-T, 135-R

0.27

0.86

5d5v_B

LushsfA2b

24

1

172-R

0.04

0.88

—

LushsfA3a

24

1

176-R

0.04

0.91

—

LushsfA3b

24

1

177-R

0.04

0.82

—

LushsfA4a

22

6

64-S, 67-R, 68-Q, 70-N, 71-T, 102-R

0.27

0.86

5d5v_B

LushsfA4b

22

6

64-S, 67-R, 68-Q, 70-N, 71-T, 102-R

0.27

0.94

5d5v_B

LushsfA4c

22

6

64-S, 67-R, 68-Q, 70-N, 71-T, 102-R

0.27

0.89

5d5v_B

LushsfA4d

22

6

64-S, 67-R, 68-Q, 70-N, 71-T, 102-R

0.27

0.84

5d5v_B

LushsfA6a

23

6

97-S, 100-R, 101-Q, 103-N, 104-T, 135-R

0.26

0.82

5d5v_B

LushsfA6b

23

6

95-S, 98-R, 99-Q, 101-N, 102-T, 133-R

0.26

0.89

5d5v_B

LushsfA7a

22

6

93-S, 96-R, 97-Q, 99-N, 100-T, 131-R

0.27

0.83

5d5v_B

LushsfA7b

22

6

93-S, 96-R, 97-Q, 99-N, 100-T, 131-R

0.27

0.87

5d5v_B

LushsfA7c

22

6

99-S, 102-R, 103-Q, 105-N, 106-T, 137-R

0.27

0.86

5d5v_B

LushsfA8a

22

6

66-S, 69-R, 70-Q, 72-N, 73-I, 104-R

0.27

0.87

5d5v_B

LushsfA8b

22

6

66-S, 69-R, 70-Q, 72-N, 73-I, 104-R

0.27

0.97

5d5v_B

LushsfA9a

22

6

183-S, 186-R, 187-Q, 189-N, 190-T, 221-R

0.27

0.89

5d5v_B

LushsfA9b

22

6

175-S, 178-R, 179-Q, 181-N, 182-T, 213-R

0.27

0.90

5d5v_B

LushsfB1a

23

6

60-S, 63-R, 64-Q, 66-N, 67-T, 98-R

0.26

0.79

5d5v_B

LushsfB1c

24

1

111-R

0.04

0.93

-

LushsfB2a

22

6

79-S, 82-R, 83-Q, 85-N, 86-T, 117-R

0.27

0.88

5d5v_B

LushsfB2b

22

6

97-S, 100-R, 101-Q, 103-N, 104-T, 135-R

0.27

0.97

5d5v_B

LushsfB2d

22

6

142-S, 145-R, 146-Q, 148-N, 149-T, 180-R

0.27

0.97

5d5v_B

LushsfB4a

23

6

83-S, 86-R, 87-Q, 89-N, 90-T, 121-R

0.26

0.98

5d5v_B

LushsfB4b

22

6

90-S, 93-R, 94-Q, 96-N, 97-T, 128-R

0.27

0.97

5d5v_B

LushsfB4c

23

6

83-S, 86-R, 87-Q, 89-N, 90-T, 121-R

0.26

0.95

5d5v_B

LushsfB5a

23

6

83-S, 86-R, 87-Q, 89-N, 90-T, 121-R

0.26

1.00

5d5v_B

LushsfB5b

23

6

84-S, 87-R, 88-Q, 90-N, 91-T, 122-R

0.26

0.99

5d5v_B

LushsfC1a

23

6

76-S, 79-R, 80-Q, 82-N, 83-T, 114-R

0.26

0.89

5d5v_B

LushsfC1b

23

6

71-S, 74-R, 75-Q, 77-N, 78-T, 109-R

0.27

0.96

5d5v_B

  1. S-serine; R-arginine, Q-glutamine, N-asparagine, T-threonine, I-isoleusine ‘–’ no model.
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