Table 4 Details of promoter analysis in the 1000 bp upstream sequence of the LusHSF genes. Position of first nucleotide of putative TSS/TATA box/Enhancer from the 5’ end of the upstream sequence analyzed and not from the start codon. NP- No prediction; PRECON70#- PRECONSCRHSP70.

From: Genomic insights into HSFs as candidate genes for high-temperature stress adaptation and gene editing with minimal off-target effects in flax

Gene_ID

Prom_len

TSSP (SOFTBERRY) predictions*

Number of cis-elements (PlantCARE)

Number of cis-elements (PLACE

TSS

TATA box

Enhancer

ABRE

CCAAT-box

DRE

HSE

LTR

MBS

TC-rich repeats

ABRE

MYB

LTRE

PRECON70#

CRT/DRE/CBF

LushsfA1a

1000

NP

2

0

0

0

0

0

1

2

13

1

1

3

LushsfA1b

1000

749

725

1

0

0

0

2

0

0

7

14

5

1

5

LushsfA1c

1000

550

512

2

0

0

1

2

0

1

1

14

2

0

0

LushsfA1d

1000

931

916

0

0

0

1

0

2

0

2

15

2

1

2

LushsfA1f

1000

450

415

0

0

0

2

0

1

1

1

21

4

2

2

LushsfA2a

1000

892

855

761

1

0

0

0

0

3

0

0

18

0

0

2

LushsfA2b

1000

NP

0

0

0

0

0

4

0

0

18

3

2

3

LushsfA3a

1000

940

927

869

2

0

0

0

0

2

1

3

24

3

2

6

LushsfA3b

1000

941

927

869

1

0

0

0

1

0

0

3

24

2

1

2

LushsfA4a

1000

NP

0

1

0

0

0

0

1

0

15

0

2

0

LushsfA4b

1000

NP

0

0

0

0

0

1

1

0

15

1

2

6

LushsfA4c

1000

NP

0

0

0

0

0

0

2

0

16

0

1

1

LushsfA4d

1000

292

256

0

0

0

1

0

1

2

0

18

1

3

6

LushsfA6a

1000

895; 461

860; 425

928

0

0

0

0

0

2

2

1

11

0

3

8

LushsfA6b

1000

905

868

926

0

0

0

0

0

1

1

0

17

1

2

7

LushsfA7a

1000

828

795

155; 874

0

0

0

1

1

0

0

0

3

1

0

0

LushsfA7b

1000

822

789

861

0

0

0

0

1

1

2

0

19

2

0

4

LushsfA7c

748

438

412

696

0

0

0

0

0

1

1

2

11

0

1

1

LushsfA8a

1000

NP

0

0

0

1

2

1

0

4

12

3

2

3

LushsfA8b

1000

NP

0

0

0

2

0

6

0

2

27

2

2

11

LushsfA9a

1000

633

616

4

0

0

6

1

1

1

4

18

2

3

2

LushsfA9b

939

NP

4

0

0

0

0

1

0

4

25

1

2

2

LushsfB1a

1000

607; 192

575; 160

615

0

0

0

0

0

0

0

1

14

1

1

0

LushsfB1c

1000

626; 175

592; 156

632

0

0

0

2

0

1

1

0

9

1

4

0

LushsfB2a

1000

NP

0

0

0

0

1

1

0

1

9

4

0

3

LushsfB2b

1000

578

540

582

2

0

0

1

1

0

2

3

12

2

0

0

LushsfB2d

1000

425

395

231

0

0

0

0

0

1

1

2

7

0

2

0

LushsfB4a

1000

223

203

922; 315

0

0

0

2

2

0

2

0

11

3

0

0

LushsfB4b

1000

NP

0

0

1

0

1

3

0

0

31

3

1

5

LushsfB4c

287

209

0

0

0

1

1

0

0

0

0

1

0

0

LushsfB5a

769

723

697

1

0

0

2

0

0

0

2

3

0

0

1

LushsfB5b

1000

945

931

1

0

0

0

0

1

0

2

24

1

1

3

LushsfC1a

1000

948

913

2

0

0

1

1

1

0

6

10

4

1

1

LushsfC1b

1000

925; 108

892; 70

936

2

0

0

0

1

3

1

3

17

2

1

4