Figure 2
From: Fetal lung C4BPA induces p100 processing in human placenta
C4BPA is contained in fetal lung exosomes. (A) Validation of differential abundance of exosomal C4BPA among groups as indicated by Western blot analysis (top panels). C4BPA abundance was normalized to FN1 and quantified with use of ImageJ program33. Student t-test was used compare either results of the TCS-A or TSL-A with those of the PCS-A. *p < 0.01 (N = 3 individual samples). (B) Venn diagram showing number of tissue-specific proteins that were obtained from the Human Protein Atlas15, indicating that C4BPA, which was identified in fetal exosomes, originates from fetal liver or lung, whereas AFP and APOH are found exclusively in liver. (C) Anti-C4BPA or -AFP antibody was used in immunoprecipitation (IP) assay of fetal exosomes prepared from the samples of 3 individual TCS-A and IPs were analyzed by means of Western blot for the listed proteins or by ELISA for identifying PAF. The bars represent average of A450 values and standard deviation from triplicates by the same IPs. Student t-test was used to compare values of A450 between α-AFP and α-C4BPA within the same sample. *p < 0.01.
