Figure 4
From: Fetal lung C4BPA induces p100 processing in human placenta
C4BPA activates non-canonical NF-κB signaling and pro-labor mediators in human placenta. (A) Term CTB were treated with the exosomes from fetal arterial umbilical cord blood at equal amount based on OD280, and protein extracts were analyzed by means of Western blot for the listed proteins. The graphs represent the ratio of p52 to p100, which were quantified with use of ImageJ program. The bars represent mean and standard deviation from experiments performed in 3 individual CTB samples by the same total exosome (exo) preparation (labeled with A, B, or C). (B) Term CTB were treated with C4BPA (Asn49–Leu597) or CD40LG, or in combination with CD40 siRNA transfection for indicated time period, protein extracts were analyzed by means of Western blot for the listed proteins. The bars represent mean and standard deviation from experiments performed in 3 individual CTB samples. (C) Term CTB were treated with C4BPA (Glu548–Leu597), or in combination with CD40 siRNA transfection for indicated time period, and protein extracts were analyzed by means of Western blot for the listed proteins. The bars represent mean and standard deviation from experiments performed in 3 individual CTB samples. *p < 0.01.
