Figure 2

Phylogenetic relationships of STEC/ETEC hybrid strains in this study to other E. coli/Shigella reference strains. The phylogenetic positions of the four hybrid strains in relative to the other 41 reference strains were studied with three different approaches: panel (A) 80% consensus tree generated from three runs of 55 ribosomal protein subunits (rps) gene ClonalFrame analysis; panel (B), Neighbor-Net phylogeny inferred from the allele profiles of the 2181 loci that shared by the 45 isolates; and panel (C) Gubbins tree of the concatenated sequences of the shared loci that found in the wgMLST analysis. The four hybrid isolates were highlighted with red letter.