Figure 5

Loss of HES1 affects BC cell viability. (A) BC cells pictured after 72 hours of transient HES1 knock-down. (B) Apoptosis in MDA-MB-231 cells after 4 days of transient HES1 knock-down. Error bars - SEM, n = 3; **p < 0,005, unpaired t-test. (C) Total length and cleaved PARP and HES1 following 3 days of transient HES1 knock-down in MCF7, MDA-MB-468 and BT-549 cell lines. Representative of two biological replicates. (D, E) Relapse-free survival (RFS) in ER-positive, PR-positive (D), and ER-negative and PR-negative (E) breast cancer. Both cohorts excluded the HER-2-amplified BCs and patients that did not receive systemic treatment. Plots built using the online KM-plotter tool²⁴.