Figure 4

Mechanical effect of SC4 on reconstituted chromatin through the specific interaction SC4/nucleosome. (A) Real time monitoring of the stiffness and viscous losses after 3 consecutive injections of water followed by 1 mM SC4 on DNA, incubation times 5 minutes each. (B) Real time monitoring of the stiffness and viscous losses after chromatin reconstitution on SNTs, and then by the consecutives injections of water, Nitric Acid (pH 3) HNO3 followed by SC4 on DNA, incubation times 5 minutes each. In black is shown the stiffness response and in red the viscous losses responses. Arrows indicate the moment of the injection. pH value of each sample is represented by the universal color code of pH paper and indicated in the inset (green for neutral pH, red for acidic pH). pH data from all samples are given in Table S1.