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Figure 1

From: Activation of immune responses against the basement membrane component collagen type IV does not affect the development of atherosclerosis in ApoE-deficient mice

Figure 1

Immunization of ApoE−/− mice with collagen α1α2(IV)-PADRE results in an IgG1 response against collagen type IV. ApoE−/− mice were immunized with collagen α1α2(IV)-PADRE peptides (25 or 50 µg), PADRE (8 or 16 µg) or PBS. Plasma from immunized mice were analyzed for IgG1 (dilution 1:5000) or IgG2c (dilution 1:500) for binding to en α1α2(IV) peptides (A,C) or PADRE (B,D) by ELISA. The specificity of IgG1 binding to collagen α1α2(IV) peptides was analyzed by addition of mouse collagen type IV protein at different concentrations (E). Kruskal-Wallis test followed by Dunn’s multiple comparisons post-test. Each dot represents one mouse and the bar denotes median value. (E) Values are represented as means of triplicates ± SD. **p < 0.005, ***p < 0.0001.

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