Figure 2 | Scientific Reports

Figure 2

From: Activation of immune responses against the basement membrane component collagen type IV does not affect the development of atherosclerosis in ApoE-deficient mice

Figure 2

Characterization of T cells in spleen of ApoE−/− mice immunized with collagen α1α2(IV)-PADRE. Splenocytes from mice immunized with collagen α1α2(IV)-PADRE peptides, PADRE or PBS were analyzed with flow cytometry. Representative flow cytometry gating strategy for CD3+CD4+, CD3+CD8+ and CD25+ FoxP3+. (A) Percentage of CD25+FoxP3+ (Treg) out of CD3+CD4+, (B) CD3+CD8+, (C) and CD3+CD4+cells, (D) for each treatment. ANOVA followed by Sidak’s multiple comparisons post-test with the bar denoting mean value. Each dot represents one mouse. *p < 0.05.

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