Table 1 List of the antibodies used for flow cytometry.
From: Maf deficiency in T cells dysregulates Treg - TH17 balance leading to spontaneous colitis
Antibody | Clone | Reference | Fluoresence Labelling | Supplier | Dilution |
|---|---|---|---|---|---|
CD25 | PC61 | 20251–83 | PE | eBioscience | 1/3000 |
CD4 | RM4–5 | 100549 | BV711 | Biolegend | 1/100 |
CD45.2 | 104 | 109835 | BV650 | Biolegend | 1/50 |
CD45RB | C363-16A | 103307 | PE | Biolegend | 1/200 |
CD8 | 53–6.7 | 100743 | BV605 | Biolegend | 1/500 |
C-MAF | sym0F1 | 50-9855-82 | eFluor 660 | eBioscience | 1/100 |
FOXP3 | FJK-16s | 48-5773-82 | eFluor 450 | eBioscience | 1/200 |
MF-14 | 126406 | Alexa Fluor 488 | Biolegend | 1/200 | |
GATA-3 | TWAJ | 46-9966-42 | PerCP-eFluor710 | eBioscience | 1/200 |
IFN-γ | XMG1.2 | 505826 | PE/Cy7 | Biolegend | 1/200 |
IL-10 | JES5-16E3 | 505007 | PE | Biolegend | 1/100 |
IL-17A | eBio17B7 | 53-7177-81 | Alexa Fluor 488 | eBioscience | 1/200 |
RORγt | B2D | 61-6981-82 | PE-eFluor 610 | eBioscience | 1/200 |
T-BET | 4B10 | 644814 | APC | Biolegend | 1/200 |
