Table 2 List of the primers used for quantitative RT-PCR.
From: Maf deficiency in T cells dysregulates Treg - TH17 balance leading to spontaneous colitis
Gene | Reverse (5′-3′) | Forward (5′-3′) |
|---|---|---|
beta2microglobulin | AGACTGATACATACGCCTGCAG | GCAGGTTCAAATGAATCTTCAG |
ifnγ | CAACAGCAAGGCGAAAAA | GGACCACTCGGATGAGCTC |
il10 | ACCTGCTCCACTGCCTTGCT | GGTTGCCAAGCCTTATCGGA |
il17a | GCTCCAGAAGGCCCTCAGA | AGCTTTCCCTCCGCATTGA |
il1b | TCGAGGCCTAATAGGCTCATCT | GCTGCTTCAGACACTTGCACAA |
il4 | GAAGCCCTACAGACGAGCTCA | GGGACGCCAT |
maf | AACATATTCCATGGCCAGGG | GGATGGCTTCAGAACTGGCA |
tnfa | TGGAAGTAGACAAGGTACAACCC | CATCTTCTCAAAATTCGAGTGACAA |
inos | CATTGGAAGTGAAGCGTTTCG | CAGCTGGGCTGTACAAACCTT |
gapdh | TCACCACCACCATGGAGAAGG | GCTAAGCAGTTGGTGGTCA |
regIIIb | QT00239302 (Quiagen) | |
regIIIg | QT00147455 (Quiagen) | |
foxp3 | QT00138369 (Quiagen) | |
