Figure 3
From: Complete assembly of the Leishmania donovani (HU3 strain) genome and transcriptome annotation
Illustration of the process followed for correcting the automatic annotation of CDS based on the transcript definition. Panel A, mapping of RNA-seq reads in the genomic region expanding the LDHU3_05.0940 gene. Panel B, mapping of SL-containing RNA-seq reads (two SL-addition sites (SASs) were mapped, the main site was covered by 72 reads and the secondary –small arrow- by only 2). Panel C, LDHU3_05.T0940 transcript annotation based on the position of the main SAS and the polyadenylation site (not shown). Panel D, automatic CDS annotation generated by Companion. Panel E, manually corrected CDS annotation, after delimiting the transcript. Panel F, proposed gene model for the LDHU3_05.0940 gene.
