Figure 6
Validation of the degradation method for downstream qPCR. (a) 260/280 absorption ratio was determined for RNA extracted from cells seeded in 2D (2D control) and from cells seeded in microdevices. No significant differences were found (Samples passed Shapiro-Wilk normality test, and Student’s t-test was performed) (p = 0.8604). (b) qPCR validation of extracted RNA. Expression fold changes in Slc2a1 (Glut-1), Ralbp1 (ralA binding protein 1) and Mki67 (Ki-67) were assessed between 24 and 72 h in samples recovered from the microdevice with the described method. Actb and Gapdh were used as reference (housekeeping) genes for normalisation. Only Mki67 yielded significantly different results from the reference value (Samples passed Shapiro-Wilk normality test and were subjected to two-way ANOVA with FDR method of Benjamini, Krieger and Yekutieli) (p = 0.012).
