Figure 5 | Scientific Reports

Figure 5

From: Advances in the role of HCV nonstructural protein 5a (NS5A) of 3a genotype in inducing insulin resistance by possible phosphorylation of AKT/PKB

Figure 5

Expression analysis of Akt/PKB gene after 1,2 and 3 hours in figure (A–C) respectively of insulin induction in transfected vs. control through Real Time PCR. HepG2 cells were transfected with pcDNA3.1/NS5A HCV construct and allowed to proliferate. Insulin induction was given for 1 hour, 2 hours and 3 hours shown in figure (A–C) respectively followed by total cellular RNA extraction and cDNA synthesis after 24hrs post transfection and relative RNA determination was carried out using Real Time PCR. GAPDH was used as internal control.

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