Figure 7

(A) Upregulation of serine 473 of Akt by NS5A gene of HCV genotype 3a at three different time intervals 1 h, 2, 3 h in control vs NS5A transfected. a: -ve control; b: control 3 h; c: control 2 h; d control 1 h; e: NS5A transfected 3 h; f: NS5A transfected 2 h; g: NS5A transfected 1 h. (B) Actin (Internal control) was confirmed in all control and transfected samples by dot bot analysis using specific antibody. a: -ve control; b: control 3 h; c: control 2 h; d: control 1 h; e: NS5A transfected 3 h; f: NS5A transfected 2 h; g: NS5A transfected 1 h.