Figure 3 | Scientific Reports

Figure 3

From: CteG is a Chlamydia trachomatis effector protein that associates with the Golgi complex of infected host cells

Figure 3

CT105 associates with the Golgi complex in C. trachomatis-infected cells. HeLa cells were infected by C. trachomatis L2/434 encoding CT105-2HA. At 19 h p.i., cells were treated for 1 h with DMSO or 1 µg/ml BFA. Then, the cells were either fixed with 4% (w/v) PFA (upper and middle panels) or washed with complete medium lacking BFA and incubated for an additional 1 h and then fixed with 4% (w/v) PFA (lower panels). Fixed cells were immunolabeled with antibodies against HA (red), C. trachomatis MOMP (gray), and GM130 (green), and appropriate fluorophore-conjugated secondary antibodies. In the area delimited by a white square (left-side panels) images were zoomed (right-side panels). All immunolabeled cells were examined by confocal fluorescence microscopy, and images correspond to single z sections. Scale bars, 10 µm.

Back to article page