Figure 7
From: Connected partner-switches control the life style of Pseudomonas aeruginosa through RpoS regulation
The HtpB/HsbRAD/RpoS signaling network. (A) Unphosphorylated form of HsbR triggers HsbA phosphorylation through its kinase/Anti-σ domain (D3 domain). Once phosphorylated HsbA binds HsbR kinase/Anti-σ factor domain and HsbD diguanylate cyclase, this binding trigger both the releases of RpoS σ-factor and c-di-GMP production which are biofilm-inducing. (B) Phosphorylated form of HsbR triggers HsbA dephosphorylation through its phosphatase domain. Once dephosphorylated HsbA binds FlgM anti-σ factor and release HsbR kinase/Anti-σ factor domain and HsbD. Under its none-phosphorylated form HsbA binds FlgM and triggers swimming motility thought FliA σ-factor. HsbA dephosphorylation shuts down biofilm formation by inhibiting HsbD c-di-GMP production and by sequestration of RpoS by HsbR.
