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Figure 2

From: Functional Analysis of Conserved Transmembrane Charged Residues and a Yeast Specific Extracellular Loop of the Plasma Membrane Na+/H+ Antiporter of Schizosaccharomyces pombe

Figure 2

Growth of S. pombe containing either wild type or SpNHE1 proteins in liquid media with concentrations of NaCl of 0, 200 mM, 500 mM or 1 M NaCl. To assess NaCl tolerance of strains media was inoculated with 2 × 106 cells into 2.5 ml of medium at 30 °C for up to 72 hours. Cell absorbance of suspensions at 600 nm was used to monitor growth at the indicated times. Results are the mean +/− SE of a minimum of three determinations. (A,B) Comparison of growth rates in NaCl medium of control and mutant strains. (C,D) Second round of mutagenesis to other amino acids or segments of SpNHE1. Ura4 refers to S. pombe with the SpNHE1 knockout described earlier8.

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