Figure 1
From: Bioproduction of pure, kilobase-scale single-stranded DNA
Scalable bacteriophage production of isogenic cssDNA. (a) Miniphage phPB52 was assembled using restriction-free (RF) cloning was used for miniphage phPB52 assembly and transformed into E. coli containing the M13cp helper plasmid for production of isogenic cssDNA. (b) aPCR was used to generate the two ssDNA megaprimers for RF cloning encoding the f1 origin of replication (f1 ori) and the bla ampicillin selection marker (Selection). See Fig. S1a for uncropped color image. (c) Phage particles from clarified media were visualized by TEM. See Fig. S1b for uncropped image. (d) DNA purification from the bacterial pellet and the clarified media show mostly pure cssDNA in the media and cssDNA and dsDNA phagemid, and helper plasmid in the bacterial pellet. See Fig. S1c for uncropped color image.
