Figure 2
From: Bioproduction of pure, kilobase-scale single-stranded DNA
Shaker flask production of pure cssDNA. (a) Shaker flask growth of phPB84 was used to optimize conditions for phage amounts and purity. (b) Time-course assay of cssDNA production of phPB84, with cssDNA yield calculated by absorbance at 280 nm and purity adjusted by agarose gel band intensity, showing maximum yield and purity at the 8-hour timepoint. The 16 h time-point is from a separate culture, and therefore is not included in the plot. See Fig. S1a for uncropped color gel image. (c) Comparison between DH5a F′Iq and SS320 showing two-fold yield increases in the SS320 strain. (d) Comparison between growth media showing five-fold improved cssDNA yield in 2 × YT after 8 hours of production. (e) Comparison of five pH values for cssDNA production, controlled by use of 100 mM HEPES-NaOH. Error bars indicate standard deviation of triplicate experiments. See Figs S3 and S3 for uncropped color triplicate measurements of all experiments.
