Table 1 Ingredients composition and proximate analysis of experimental diets.

From: Selection of carbohydrate-active probiotics from the gut of carnivorous fish fed plant-based diets

Dietsa

1st Trial Diets

2nd Trial Diets

CTR

SBM

RSM

SFM

CTR−

FI99

FI162

Mix

CTR+

Ingredients (% dry weight)

Fish mealb

60.2

38.7

45.2

48.1

5.0

5.0

5.0

5.0

24.5

Fish protein concentc

─

─

─

─

5.0

5.0

5.0

5.0

5.0

Pea protein concentd

─

─

─

─

4.2

4.2

4.2

4.2

5.0

Soy bean meale

─

30.0

─

─

20.0

20.0

20.0

20.0

10.0

Rapeseed mealf

─

─

30.0

─

10.0

10.0

10.0

10.0

─

Sunflower mealg

─

─

─

30.0

─

─

─

─

─

Wheat mealh

─

─

─

─

8.2

8.2

8.2

8.2

20.0

Wheat gluteni

─

─

─

─

10.0

10.0

10.0

10.0

5.0

Corn glutenj

─

─

─

─

15.0

15.0

15.0

15.0

10.0

Pregelat. maize starchk

23.2

11.6

8.0

4.8

─

─

─

─

─

Fish oil

12.1

13.6

12.4

13.0

14.6

14.6

14.6

14.6

13.0

Bicalcium phosphatel

1.0

2.6

1.0

0.6

3.1

3.1

3.1

3.1

3.5

Choline chloride (50%)

0.5

0.5

0.5

0.5

0.5

0.5

0.5

0.5

0.5

Vitamin premixm

1.0

1.0

1.0

1.0

1.0

1.0

1.0

1.0

1.0

Mineral premixn

1.0

1.0

1.0

1.0

1.0

1.0

1.0

1.0

1.0

Binder°

1.0

1.0

1.0

1.0

1.0

1.0

1.0

1.0

1.0

L-lysinep

─

─

─

─

0.6

0.6

0.6

0.6

—

L-methioninep

─

─

─

─

0.5

0.5

0.5

0.5

0.4

Taurinep

─

─

─

─

0.3

0.3

0.3

0.3

0.2

Proximate analysis (% dry weight)

Dry matter

91.5

92.4

92.7

93.5

91.5

92.0

92.0

91.0

92.6

Crude protein

46.9

46.5

46.3

46.4

44.9

46.5

45.7

46.3

44.9

Crude lipids

17.3

16.1

16.6

16.8

18.1

18.7

18.5

17.8

17.8

Ash

11.3

11.7

11.3

11.1

8.2

8.0

8.0

8.2

8.7

  1. aCTR, control fishmeal based diet; SBM, soybean meal based diet; RSM, rapeseed meal based diet; SFM, sunflower meal based diet; CTR−, negative control plant-feedstuffs based diet; FI99, FI99 probiotic-enriched diet; FI162, FI162 probiotic-enriched diet; Mix, FI99 + FI162 enriched diet, CTR+, positive control fishmeal based diet. bSteam Dried LT-FM: 1st Trial Diets from Pesquera Diamante, Austral Group, S.A Perú (CP: 74.7% DM; GL: 9.8% DM); 2nd Trial Diets from Copicesa S. A., Spain (CP: 77.1% DM; GL: 11.8% DM). cFish protein concentrate: Sopropèche G, France (CP: 77.0% DM; GL: 18.4% DM). dPea protein concentrate: Sorgal, S.A.Ovar, Portugal (CP: 52.5%; CL: 5.9%). eSorgal, S.A.Ovar, Portugal: 1st Trial Diets (CP: 53.7% DM; GL: 2.1% DM); 2nd Trial Diets (CP: 52.0% DM; GL: 1.9% DM). fSorgal, S.A.Ovar, Portugal: 1st Trial Diets (CP: 37.5% DM; GL: 4.0% DM); 2nd Trial Diets (CP: 37.3% DM; GL: 5.0% DM). gSorgal, S.A.Ovar, Portugal (CP: 30.3% DM; GL: 1.0% DM). hSorgal, S.A. Ovar, Portugal (CP: 14.5% DM; GL: 2.0% DM). iSorgal, S.A. Ovar, Portugal (CP: 83.1% DM; GL: 1.4% DM). jSorgal, S.A. Ovar, Portugal (CP: 70.1% DM; GL: 2.8% DM). kPregelatinized maize starch C-Gel Instant-12016, Cerestar, Mechelen, Belgium. lPremix, Portugal (Calcium: 24%; Total phosphorus: 18%). mVitamins (mg kg−1 diet): retinol acetate, 18,000 (IU kg−1 diet); cholecalciferol, 2000 (IU kg−1 diet); alfa tocopherol acetate, 35; sodium menadione bisulphate, 10; thiamine-HCl, 15; riboflavin, 25; calcium pantothenate, 50; nicotinic acid, 200; pyridoxine HCl, 5; folic acid, 10; cyanocobalamin, 0.02; biotin, 1.5; ascorbic acid, 50; inositol, 400. nMinerals (mg kg−1 diet): cobalt sulphate, 1.91; copper sulphate, 19.6; iron sulphate, 200; sodium fluoride, 2.21; potassium iodide, 078; magnesium oxide, 830; manganese oxide, 26; sodium selenite, 0.66; zinc oxide, 37.5; dibasic calcium phosphate, 8.02 (g kg−1 diet); potassium chloride, 1.15 (g kg−1 diet); sodium chloride, 0.44 (g kg−1 diet). jAquacube (guar gum, polymethyl carbamide, manioc starch blend, hydrate calcium sulphate) Agil, UK. p Feed-grade, Sorgal, S.A. Ovar, Portugal.
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