Table 1 Methylation in relation to life stage and sex. Linear mixed models of methylation at the six loci in relation to life stage (adult vs nestling) and sex. In the models we included year, colony and family as random effects. The interaction term between life stage and sex was removed when its effect was statistically non-significant before FDR. Thus, for example, the main effects of life stage and sex on clock poly-Q methylation are computed while excluding from the model the interaction effect, as indicated by the asterisk. See Fig. 2 for mean within-group values. n = sample size.

From: Inter-generational resemblance of methylation levels at circadian genes and associations with phenology in the barn swallow

 

F

df

P

Clock 5′-UTR (n = 245)

   Life stage

19.81

1,183

<0.001

   Sex

4.53

1,183

0.035

   Life stage × Sexa

7.82

1,183

0.006

Clock poly-Q (n = 242)

   Life stage

19.00

1,182

<0.001

   Sex

24.14

1,182

<0.001

   Life stage × Sex*

3.92

1,181

0.049

Cry1 (n = 229)

   Life stage

2.50

1,169

0.116

   Sex

2.46

1,169

0.118

   Life stage × Sex*

0.00

1,168

0.997

Per2 (n = 238)

   Life stage

0.35

1,178

0.514

   Sex

0.74

1,178

0.392

   Life stage × Sex*

0.12

1,177

0.728

Per3 (n = 243)

   Life stage

4.32

1,183

0.039

   Sex

2.99

1,183

0.085

   Life stage × Sex*

0.37

1,182

0.542

Timeless (n = 191)

   Life stage

38.84

1,131

<0.001

   Sex

15.99

1,131

0.001

   Life stage × Sex*

0.15

1,130

0.697

  1. aAdult females > nestling females; nestling males > nestling females at post hoc tests (P < 0.009); *Interaction term excluded from the model including main effects.
  2. Indicates statistical significance after FDR correction; the P-values reported in the table are those estimated before FDR correction.